Understanding Energetics of Enlite™ Chemiluminescence with Recombinant Protein Analysis
The Enlite™ Chemiluminescence system by Invitrogen is a powerful tool used to detect proteins in biological samples. It employs recombinant protein technology to produce a stable signal that is highly sensitive, selective, and reproducible. In this article, we will explore the principles of Enlite™ Chemiluminescence and how it is used in recombinant protein analysis.
Enlite™ Chemiluminescence principle
The Enlite™ Chemiluminescence system is based on the detection of horseradish peroxidase (HRP) enzyme, which reacts with a luminol substrate in the presence of hydrogen peroxide. This reaction produces a highly stable light signal that can be measured using a luminometer. The Enlite™ system uses recombinant technology to produce an HRP-luminol conjugate that has a high specific activity and low background noise, which greatly enhances sensitivity and selectivity.
The Enlite™ Chemiluminescence system is highly adaptable and can be used for a wide range of applications, including western blot, ELISA, and protein detection in complex mixtures such as cell lysates. It is also highly compatible with HRP-conjugated primary and secondary antibodies, making it an ideal choice for protein detection in immunoprecipitation and pull-down assays.
Recombinant protein analysis using Enlite™ Chemiluminescence
Recombinant protein analysis is a powerful technique that is used to study protein-protein interactions, protein folding, and function. Enlite™ Chemiluminescence has revolutionized recombinant protein analysis, providing researchers with an easy-to-use and highly sensitive tool for studying proteins in vitro.
The Enlite™ system can be used to analyze recombinant proteins in a range of applications, including protein purification, protein-protein interactions, and phosphorylation studies. For example, Enlite™ Chemiluminescence can be used to detect recombinant His-tagged proteins expressed in bacterial or mammalian cells. In addition, Enlite™ Chemiluminescence can be used to analyze protein-protein interactions using recombinant fusion proteins such as glutathione-S-transferase (GST)-tagged proteins.
Conclusion
The Enlite™ Chemiluminescence system is a powerful tool for recombinant protein analysis in vitro. It utilizes recombinant technology to produce a stable and highly sensitive light signal that can be used to detect proteins in biological samples. Enlite™ Chemiluminescence is highly adaptable and can be used for a range of applications, making it a valuable tool for research in molecular biology, biochemistry, and biotechnology.
